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Blotting techniques are increasingly gaining importance globally that is used to separate and identify the proteins. Blocking buffers are one of the techniques that are used for this purpose. Blocking buffers are composed of slat solutions which include tris-buffered saline (TBS; 50 mM Tris and 150 mM NaCl, pH 7.6) and phosphate-buffered saline (PBS; 140 mM NaCl, 10 mM phosphate buffer, and 3 mM KCl, pH 7.4).

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