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When it comes to urine sediment examination there are several manual methods to choose from

There are several manual methods for urine sediment examination, including counting urine analyzer sediment in a standardized or non-standardized manner under a coverslip, counting centrifuged or uncentrifuged https://www.chinacaremedical.com/c/urine-analyzer_0429 microscopic urine analysis specimens in a chamber, and counting urine sediment under a coverslip. It has been common practice in many laboratories to perform traditional (non-standardized) urine analyzer sediment analysis. The non-standardized sediment procedure, on the other hand, was not recommended due to the high degree of uncertainty in the results and the reduced sensitivity in detecting essential formed elements. As a routine visual procedure for kidney-related urine formed elements, it is recommended to use a standardized microscopic urine analysis sediment examination under a coverslip rather than a non-standardized urine sediment examination under a coverslip. A reference method for urine analyzer microscopy should be capable of both accurate identification of the various formed elements and accurate quantification of those elements. Currently, there is no such method available. Using bright-field microscopy of unstained preparations for particle counting is insufficient for the detection of bacteria, erythrocytes, and hyaline casts, among other things. As a result, either supravital staining or phase-contrast microscopy, or a combination of both, is recommended for better examination 1. Phase-contrast microscopy, on the other hand, is not available in every laboratory.The following factors can influence the outcome of the microscopic examination of urine: the speed and duration of centrifugation, the amount of urine remaining in the tube for resuspension, and whether or not the urine is stained. Manual microscopic examination necessitates the use of highly trained and experienced personnel and requires a significant amount of time. As a result, automatic urine sediment analyzers for high-volume laboratories were developed in order to improve standardization, increase measurement certainty, and save time for laboratory personnel.The introduction of automated microscopes and flow cytometry devices, which were inspired by blood cell counting, marked the beginning of the methodology of microscopic urine analysis particle analysis 1. Specifically, these analyzers make use of two analytical principles for  sediment analysis: one based on electrical impedance and another requiring the use of computerized imaging and classification systems that sort particles according to preset particle dimensions. At this point, it is unclear which principle is superior to the others.The image-based analysis systems scan the formed elements of flowing  and display the images of the formed elements on a computer screen in an automated fashion. Before the results of the analysis are reported, the shaped elements must be visually examined by well-trained personnel who can then decide whether to approve, delete, or reclassify them. However, laboratories that have made the transition from the manual microscopic method to automatic systems continue to have some reservations about the consistency of the results they are receiving.The use of automated analyzers (such as the Iris iQ200 ELITE and the Dirui FUS-200) in medical laboratories has steadily increased over time. There has been no published study that compares the two analyzers, which are both image-based analysis systems. The concordance between manual microscopy and the two automatic urine sediment analyzers was tested for the first time.

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